Molecular and immunological characterization of the glycosylated orange allergen Cit s 1
Identifieur interne : 002348 ( Main/Exploration ); précédent : 002347; suivant : 002349Molecular and immunological characterization of the glycosylated orange allergen Cit s 1
Auteurs : Gerald Pltl [Autriche] ; Oussama Ahrazem [Espagne] ; Katharina Paschinger [Autriche] ; M. Dolores Ibaez [Espagne] ; Gabriel Salcedo [Espagne] ; Iain B. H. Wilson [Autriche]Source :
- Glycobiology [ 0959-6658 ] ; 2007-02.
English descriptors
- KwdEn :
- Allergens (chemistry), Allergens (immunology), Amino Acid Sequence, Citrus sinensis (immunology), Cross Reactions, Enzyme-Linked Immunosorbent Assay, Fucose (analysis), Glycoproteins (chemistry), Glycoproteins (immunology), Glycosylation, Humans, Immune Sera (immunology), Immunoglobulin E (blood), Mass Spectrometry, Molecular Sequence Data, Peptide Fragments (chemistry), Plant Proteins (chemistry), Plant Proteins (immunology), Polysaccharides (chemistry), Polysaccharides (immunology), Sequence Analysis, Protein, Trypsin (chemistry), Xylose (analysis).
- MESH :
- chemical , analysis : Fucose, Xylose.
- chemical , blood : Immunoglobulin E.
- chemical , chemistry : Allergens, Glycoproteins, Peptide Fragments, Plant Proteins, Polysaccharides, Trypsin.
- chemical , immunology : Allergens, Glycoproteins, Immune Sera, Plant Proteins, Polysaccharides.
- immunology : Citrus sinensis.
- Amino Acid Sequence, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Glycosylation, Humans, Mass Spectrometry, Molecular Sequence Data, Sequence Analysis, Protein.
Abstract
The IgE of sera from patients with a history of allergy to oranges (Citrus sinensis) binds a number of proteins in orange extract, including Cit s 1, a germin-like protein. In the present study, we have analyzed its immunological cross-reactivity and its molecular nature. Sera from many of the patients examined recognize a range of glycoproteins and neoglycoconjugates containing 1,2-xylose and core 1,3-fucose on their N-glycans. These reagents also inhibited the interaction of Cit s 1 with patients' sera, thus underlining the critical role of glycosylation in the recognition of this protein by patients' IgE and extending previous data showing that deglycosylated Cit s 1 does not possess IgE epitopes. In parallel, we examined the peptide sequence and glycan structure of Cit s 1, using mass spectrometric techniques. Indeed, we achieved complete sequence coverage of the mature protein compared with the translation of an expressed sequence tag cDNA clone and demonstrated that the single N-glycosylation site of this protein carries oligosaccharides with xylose and fucose residues. Owing to the presumed requirement for multivalency for in vivo allergenicity, our molecular data showing that Cit s 1 is monovalent as regards glycosylation and that the single N-glycan is the target of the IgE response to this protein explain the immunological cross-reactive properties of Cit s 1 as well as its equivocal nature as a clinically relevant allergen.
Url:
- https://api.istex.fr/document/EDEA3504B402F35FDE423511061506F9B887B45D/fulltext/pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848330
DOI: 10.1093/glycob/cwl068
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000679
- to stream Istex, to step Curation: 000679
- to stream Istex, to step Checkpoint: 000626
- to stream Pmc, to step Corpus: 000D76
- to stream Pmc, to step Curation: 000D75
- to stream Pmc, to step Checkpoint: 001138
- to stream PubMed, to step Corpus: 000B55
- to stream PubMed, to step Curation: 000B55
- to stream PubMed, to step Checkpoint: 000B55
- to stream Ncbi, to step Merge: 000642
- to stream Ncbi, to step Curation: 000642
- to stream Ncbi, to step Checkpoint: 000642
- to stream Main, to step Merge: 002432
- to stream Main, to step Curation: 002348
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Molecular and immunological characterization of the glycosylated orange allergen Cit s 1</title><author><name sortKey="Pltl, Gerald" sort="Pltl, Gerald" uniqKey="Pltl G" first="Gerald" last="Pltl">Gerald Pltl</name></author><author><name sortKey="Ahrazem, Oussama" sort="Ahrazem, Oussama" uniqKey="Ahrazem O" first="Oussama" last="Ahrazem">Oussama Ahrazem</name></author><author><name sortKey="Paschinger, Katharina" sort="Paschinger, Katharina" uniqKey="Paschinger K" first="Katharina" last="Paschinger">Katharina Paschinger</name></author><author><name sortKey="Ibaez, M Dolores" sort="Ibaez, M Dolores" uniqKey="Ibaez M" first="M. Dolores" last="Ibaez">M. Dolores Ibaez</name></author><author><name sortKey="Salcedo, Gabriel" sort="Salcedo, Gabriel" uniqKey="Salcedo G" first="Gabriel" last="Salcedo">Gabriel Salcedo</name></author><author><name sortKey="Wilson, Iain B H" sort="Wilson, Iain B H" uniqKey="Wilson I" first="Iain B. H." last="Wilson">Iain B. H. Wilson</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:EDEA3504B402F35FDE423511061506F9B887B45D</idno><date when="2007" year="2007">2007</date><idno type="doi">10.1093/glycob/cwl068</idno><idno type="url">https://api.istex.fr/document/EDEA3504B402F35FDE423511061506F9B887B45D/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000679</idno><idno type="wicri:Area/Istex/Curation">000679</idno><idno type="wicri:Area/Istex/Checkpoint">000626</idno><idno type="wicri:doubleKey">0959-6658:2007:Pltl G:molecular:and:immunological</idno><idno type="wicri:source">PMC</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848330</idno><idno type="RBID">PMC:2848330</idno><idno type="wicri:Area/Pmc/Corpus">000D76</idno><idno type="wicri:Area/Pmc/Curation">000D75</idno><idno type="wicri:Area/Pmc/Checkpoint">001138</idno><idno type="wicri:source">PubMed</idno><idno type="wicri:Area/PubMed/Corpus">000B55</idno><idno type="wicri:Area/PubMed/Curation">000B55</idno><idno type="wicri:Area/PubMed/Checkpoint">000B55</idno><idno type="wicri:Area/Ncbi/Merge">000642</idno><idno type="wicri:Area/Ncbi/Curation">000642</idno><idno type="wicri:Area/Ncbi/Checkpoint">000642</idno><idno type="wicri:doubleKey">0959-6658:2006:Poltl G:molecular:and:immunological</idno><idno type="wicri:Area/Main/Merge">002432</idno><idno type="wicri:Area/Main/Curation">002348</idno><idno type="wicri:Area/Main/Exploration">002348</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Molecular and immunological characterization of the glycosylated orange allergen Cit s 1</title><author><name sortKey="Pltl, Gerald" sort="Pltl, Gerald" uniqKey="Pltl G" first="Gerald" last="Pltl">Gerald Pltl</name><affiliation wicri:level="1"><country xml:lang="fr">Autriche</country><wicri:regionArea>Department fr Chemie, Universitt fr Bodenkultur, Muthgasse 18, A-1190 Wien</wicri:regionArea><wicri:noRegion>A-1190 Wien</wicri:noRegion></affiliation></author><author><name sortKey="Ahrazem, Oussama" sort="Ahrazem, Oussama" uniqKey="Ahrazem O" first="Oussama" last="Ahrazem">Oussama Ahrazem</name><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Unidad de Bioqumica, Departamento de Biotecnologa, ETS Ingenieros Agrnomos, UPM, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Paschinger, Katharina" sort="Paschinger, Katharina" uniqKey="Paschinger K" first="Katharina" last="Paschinger">Katharina Paschinger</name><affiliation wicri:level="1"><country xml:lang="fr">Autriche</country><wicri:regionArea>Department fr Chemie, Universitt fr Bodenkultur, Muthgasse 18, A-1190 Wien</wicri:regionArea><wicri:noRegion>A-1190 Wien</wicri:noRegion></affiliation></author><author><name sortKey="Ibaez, M Dolores" sort="Ibaez, M Dolores" uniqKey="Ibaez M" first="M. Dolores" last="Ibaez">M. Dolores Ibaez</name><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Servicio de Alergia, Hospital Universitario Nio Jess, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Salcedo, Gabriel" sort="Salcedo, Gabriel" uniqKey="Salcedo G" first="Gabriel" last="Salcedo">Gabriel Salcedo</name><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Unidad de Bioqumica, Departamento de Biotecnologa, ETS Ingenieros Agrnomos, UPM, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Wilson, Iain B H" sort="Wilson, Iain B H" uniqKey="Wilson I" first="Iain B. H." last="Wilson">Iain B. H. Wilson</name><affiliation wicri:level="1"><country xml:lang="fr">Autriche</country><wicri:regionArea>Department fr Chemie, Universitt fr Bodenkultur, Muthgasse 18, A-1190 Wien</wicri:regionArea><wicri:noRegion>A-1190 Wien</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Autriche</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Glycobiology</title><idno type="ISSN">0959-6658</idno><idno type="eISSN">1460-2423</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2007-02">2007-02</date><biblScope unit="volume">17</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="220">220</biblScope><biblScope unit="page" to="230">230</biblScope></imprint><idno type="ISSN">0959-6658</idno></series><idno type="istex">EDEA3504B402F35FDE423511061506F9B887B45D</idno><idno type="DOI">10.1093/glycob/cwl068</idno><idno type="ArticleID">cwl068</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0959-6658</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Allergens (chemistry)</term><term>Allergens (immunology)</term><term>Amino Acid Sequence</term><term>Citrus sinensis (immunology)</term><term>Cross Reactions</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Fucose (analysis)</term><term>Glycoproteins (chemistry)</term><term>Glycoproteins (immunology)</term><term>Glycosylation</term><term>Humans</term><term>Immune Sera (immunology)</term><term>Immunoglobulin E (blood)</term><term>Mass Spectrometry</term><term>Molecular Sequence Data</term><term>Peptide Fragments (chemistry)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (immunology)</term><term>Polysaccharides (chemistry)</term><term>Polysaccharides (immunology)</term><term>Sequence Analysis, Protein</term><term>Trypsin (chemistry)</term><term>Xylose (analysis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Fucose</term><term>Xylose</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Immunoglobulin E</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Allergens</term><term>Glycoproteins</term><term>Peptide Fragments</term><term>Plant Proteins</term><term>Polysaccharides</term><term>Trypsin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Allergens</term><term>Glycoproteins</term><term>Immune Sera</term><term>Plant Proteins</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Cross Reactions</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Glycosylation</term><term>Humans</term><term>Mass Spectrometry</term><term>Molecular Sequence Data</term><term>Sequence Analysis, Protein</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">The IgE of sera from patients with a history of allergy to oranges (Citrus sinensis) binds a number of proteins in orange extract, including Cit s 1, a germin-like protein. In the present study, we have analyzed its immunological cross-reactivity and its molecular nature. Sera from many of the patients examined recognize a range of glycoproteins and neoglycoconjugates containing 1,2-xylose and core 1,3-fucose on their N-glycans. These reagents also inhibited the interaction of Cit s 1 with patients' sera, thus underlining the critical role of glycosylation in the recognition of this protein by patients' IgE and extending previous data showing that deglycosylated Cit s 1 does not possess IgE epitopes. In parallel, we examined the peptide sequence and glycan structure of Cit s 1, using mass spectrometric techniques. Indeed, we achieved complete sequence coverage of the mature protein compared with the translation of an expressed sequence tag cDNA clone and demonstrated that the single N-glycosylation site of this protein carries oligosaccharides with xylose and fucose residues. Owing to the presumed requirement for multivalency for in vivo allergenicity, our molecular data showing that Cit s 1 is monovalent as regards glycosylation and that the single N-glycan is the target of the IgE response to this protein explain the immunological cross-reactive properties of Cit s 1 as well as its equivocal nature as a clinically relevant allergen.</div></front></TEI><affiliations><list><country><li>Autriche</li><li>Espagne</li></country><region><li>Communauté de Madrid</li></region><settlement><li>Madrid</li></settlement></list><tree><country name="Autriche"><noRegion><name sortKey="Pltl, Gerald" sort="Pltl, Gerald" uniqKey="Pltl G" first="Gerald" last="Pltl">Gerald Pltl</name></noRegion><name sortKey="Paschinger, Katharina" sort="Paschinger, Katharina" uniqKey="Paschinger K" first="Katharina" last="Paschinger">Katharina Paschinger</name><name sortKey="Wilson, Iain B H" sort="Wilson, Iain B H" uniqKey="Wilson I" first="Iain B. H." last="Wilson">Iain B. H. Wilson</name><name sortKey="Wilson, Iain B H" sort="Wilson, Iain B H" uniqKey="Wilson I" first="Iain B. H." last="Wilson">Iain B. H. Wilson</name></country><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Ahrazem, Oussama" sort="Ahrazem, Oussama" uniqKey="Ahrazem O" first="Oussama" last="Ahrazem">Oussama Ahrazem</name></region><name sortKey="Ibaez, M Dolores" sort="Ibaez, M Dolores" uniqKey="Ibaez M" first="M. Dolores" last="Ibaez">M. Dolores Ibaez</name><name sortKey="Salcedo, Gabriel" sort="Salcedo, Gabriel" uniqKey="Salcedo G" first="Gabriel" last="Salcedo">Gabriel Salcedo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002348 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002348 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:EDEA3504B402F35FDE423511061506F9B887B45D
|texte= Molecular and immunological characterization of the glycosylated orange allergen Cit s 1
}}
| This area was generated with Dilib version V0.6.25. |  |